This pilot study investigates the safety and feasibility of 3D ultrasound-guided transperineal microwave ablation (TMA) for treating benign prostatic hyperplasia (BPH) in 12 men. The participants had a prostate size of 30-80 ml and IPSS scores of ≥14. The procedure was performed under various anesthesia methods, using the Koelis Trinity 3D transrectal ultrasound for accurate needle placement. All patients underwent successful ablation with a median of four treatments each. While there were some mild adverse events, including hematuria and prolonged catheterization, TMA was deemed safe and effective, demonstrating promising outcomes in symptom relief over six months.