This study explores the use of hyaluronic acid combined with amino acids (HAAM) for facial skin rejuvenation in aesthetic medicine. Hyaluronic acid is a popular ingredient known to enhance moisture and support extracellular matrix molecules, thereby contributing to anti-aging effects. The research focused on assessing the effects of low molecular weight hyaluronic acid fragments combined with amino acids when delivered via intradermal microinjections. A group of twenty women, aged between 35 and 64 years (average age 45), participated in the study; among them, 8 were menopausal, and 12 were within childbearing age. Mesotherapy was the chosen technique to administer HAAM to these patients. To evaluate the treatment’s effects, each participant underwent circular punch biopsies both before and three months after the treatment. Additionally, ultrasound examinations were performed using B-mode imaging with frequencies from 5 to 13 MHz to capture 2D images in both longitudinal and transverse orientations. Sixty ultrasound examinations were conducted in total, with thirty taken before and thirty after the HAAM treatment.
The histological findings showed a notable increase in fibroblast activity, leading to the production of Type III reticular collagen, an increase in blood vessel formation, and greater epidermal thickness in the treated areas. Despite these promising histological changes, the ultrasound analysis indicated no significant difference in skin thickness in specific facial areas, including the malar region, chin, and mandibular angle. These results suggest that while HAAM treatment has a substantial impact at the cellular level, visible structural changes in skin thickness may not always be detectable by ultrasound in the short term. Overall, the study highlights the potential of HAAM for enhancing skin rejuvenation by improving dermal composition, even if measurable changes in skin thickness are limited.
